Short communicationDesign and validation of a real-time RT-PCR for the simultaneous detection of enteroviruses and parechoviruses in clinical samples
Section snippets
Competing interests
None declared.
Authors’ contribution
Study concept and design: M. Cabrerizo, C. Calvo, N. Rabella, C. Muñoz-Almagro, E. del Amo, M. Pérez-Ruiz, S. Sanbonmatsu, A. Moreno-Docón, M.L.G. Trallero, and the study group of “Enterovirus and parechovirus infections in children under 3 y, Spain” PI12-00904 (see Appendix A). Contribution of samples and clinical data: C. Calvo, N. Rabella, C. Muñoz-Almagro, E. del Amo, M. Pérez-Ruiz, S. Sanbonmatsu, A. Moreno-Docón, and the study group of “Enterovirus and parechovirus infections in children
Ethical approval
An informed consent form was obtained from the parents at the time of each child's hospitalisation. Samples were anonymized and archived according to the research project protocol PI12-00904 approved by the Research Ethics and Animal Welfare Committee of National Health Institute “Carlos III”.
Acknowledgements
We thank Isidoro Bustillo, Hortensia del Pozo and Puri Higueras for their technical assistance and Ingrid M. Outschoorn for English editing.
The project was supported from a grant from the Health Research System (AES; PI12-00904).
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- 1
The members of the study group are listed in Appendix A.