Elsevier

Methods

Volume 158, 1 April 2019, Pages 61-68
Methods

Multiplexed detection and identification of respiratory pathogens using the NxTAG® respiratory pathogen panel

https://doi.org/10.1016/j.ymeth.2019.01.005Get rights and content

Highlights

  • NxTAG chemistry offers an easy workflow, low hands-on-time and scalable throughput.

  • All post-extraction assay steps take place in a single, closed tube.

  • Clinical performance of NxTAG RPP is comparable to that of the xTAG RVP assay.

  • Analytical performance is also similar or better than xTAG and xTAG FAST assays.

Abstract

The Luminex® NxTAG® Respiratory Pathogen Panel (NxTAG RPP) is an IVD-cleared assay for the simultaneous detection and identification of nucleic acids from 18 respiratory viruses and 2 (or 3 outside of the U.S.) atypical bacterial pathogens in nasopharyngeal swabs. Its scalability allows concurrent testing of up to 96 samples in a single batch. Nucleic acid extracted from 200 µL of raw specimen using the easyMAG® extractor is added directly to pre-plated, lyophilized bead reagents (LBRs), where multiplexed RT-PCR and hybridization to MagPlex-TAG™ microspheres occurs within a sealed reaction well using a single cycling program. Data acquisition is done on the MAGPIX® instrument which reads and sorts the reaction products directly from the sealed well following transfer of the assay plate from the thermal cycler. NxTAG is the newest innovation in bead-based nucleic acid chemistry developed by Luminex. Here we provide the detailed assay protocol and present data which describe the clinical and analytical performance characteristics of NxTAG RPP.

Keywords

Luminex
Multiplex
NxTAG
Respiratory pathogen
Bead array
IVD

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